采用液相色谱-串联质谱法检测可卡因及其代谢物。

Cocaine and metabolites by LC-MS/MS.

作者信息

Snozek Christine L H, Bjergum Matthew W, Langman Loralie J

机构信息

Department of Laboratory Medicine, Mayo Clinic, Rochester, MN, USA.

出版信息

Methods Mol Biol. 2012;902:91-103. doi: 10.1007/978-1-61779-934-1_8.

DOI:10.1007/978-1-61779-934-1_8

PMID:22767110

Abstract

Abuse of the stimulant cocaine (COC) is a common problem in the United States and elsewhere. The drug can be used either as the powder or as the free base (crack COC), and causes feelings of alertness and euphoria; both forms of COC are powerfully addictive. The assay described here is designed to detect and quantitate parent COC, its major metabolite benzoylecgonine, and a selection of metabolites that can provide specific information about sample validity (m-hydroxybenzoylecgonine), potential toxicity (norcocaine), route of administration (anhydroecgonine methyl ester), and co-utilization with ethanol (cocaethylene).

摘要

滥用兴奋剂可卡因（COC）在美国及其他地区是一个常见问题。该药物可以以粉末形式或游离碱形式（快克可卡因）使用，会使人产生警觉感和欣快感；两种形式的可卡因都极易成瘾。此处描述的检测方法旨在检测和定量可卡因母体、其主要代谢物苯甲酰爱康宁，以及一系列能够提供有关样本有效性（间羟基苯甲酰爱康宁）、潜在毒性（去甲可卡因）、给药途径（脱水爱康宁甲酯）和与乙醇共同使用情况（可口乙酮）的特定信息的代谢物。