The Blood Bank, Landspitali University Hospital, Reykjavik, Iceland.
J Biomed Mater Res A. 2012 Dec;100(12):3392-9. doi: 10.1002/jbm.a.34283. Epub 2012 Jul 6.
Clinical treatment of orthopaedic tissue injuries often involves the use of titanium and titanium alloys with considerable research focusing on the surface modification of these materials. Chitosan, the partly deacetylated form of chitin, is one of the materials under investigation as surface coating for orthopaedic implants in order to improve osteo-integration and cellular attachment. In this study, we determined the effects of the degree of deacetylation (DD) of chitosan membranes on attachment, proliferation and osteogenic differentiation of MC3T3-E1 mouse preosteoblasts. Chitosan membranes were coated with fibronectin to promote biocompatibility and cellular attachment. Membranes were characterized in terms of wettability and surface topography using water contact angle measurements and atomic force microscopy. The results in this study indicate that the surface roughness and fibronectin adsorption increase with increased DD. A higher DD also facilitates attachment and proliferation of cells, but no induction of spontaneous osteogenic differentiation was observed. Lower DD chitosan membranes were successfully prepared to sustain attachment and were modified by crosslinking with glutaraldehyde to promote long-term studies. The chitosan membranes used in this study are suitable as a potential coating for titanium implants.
临床治疗骨科组织损伤时通常会用到钛及钛合金，而大量研究都集中在这些材料的表面改性。壳聚糖是甲壳素部分脱乙酰化的产物，是一种研究中的骨科植入物表面涂层材料，可改善骨整合和细胞附着。在本研究中，我们测定了壳聚糖膜的脱乙酰度（DD）对 MC3T3-E1 小鼠前成骨细胞附着、增殖和成骨分化的影响。壳聚糖膜用纤连蛋白涂覆以提高生物相容性和细胞附着。通过水接触角测量和原子力显微镜对膜的润湿性和表面形貌进行了表征。本研究结果表明，表面粗糙度和纤连蛋白吸附随 DD 的增加而增加。较高的 DD 还促进了细胞的附着和增殖，但未观察到自发成骨分化的诱导。成功制备了较低 DD 的壳聚糖膜以维持附着，并通过戊二醛交联进行改性以促进长期研究。本研究中使用的壳聚糖膜适合用作钛植入物的潜在涂层。
