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鉴定和表征参与丁酸梭菌丁醇耐受性的两个功能未知基因。

Identification and characterization of two functionally unknown genes involved in butanol tolerance of Clostridium acetobutylicum.

机构信息

Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

出版信息

PLoS One. 2012;7(6):e38815. doi: 10.1371/journal.pone.0038815. Epub 2012 Jun 29.

DOI:10.1371/journal.pone.0038815
PMID:22768047
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3387226/
Abstract

Solvents toxicity is a major limiting factor hampering the cost-effective biotechnological production of chemicals. In Clostridium acetobutylicum, a functionally unknown protein (encoded by SMB_G1518) with a hypothetical alcohol interacting domain was identified. Disruption of SMB_G1518 and/or its downstream gene SMB_G1519 resulted in increased butanol tolerance, while overexpression of SMB_G1518-1519 decreased butanol tolerance. In addition, SMB_G1518-1519 also influences the production of pyruvate:ferredoxin oxidoreductase (PFOR) and flagellar protein hag, the maintenance of cell motility. We conclude that the system of SMB_G1518-1519 protein plays a role in the butanol sensitivity/tolerance phenotype of C. acetobutylicum, and can be considered as potential targets for engineering alcohol tolerance.

摘要

溶剂毒性是阻碍化学物质经济高效的生物技术生产的主要限制因素。在丙酮丁醇梭菌中，鉴定到一个功能未知的蛋白（由 SMB_G1518 编码），该蛋白具有假定的醇相互作用结构域。SMB_G1518 和/或其下游基因 SMB_G1519 的缺失导致丁醇耐受性增加，而过表达 SMB_G1518-1519 则降低了丁醇耐受性。此外，SMB_G1518-1519 还影响丙酮酸：铁氧还蛋白氧化还原酶（PFOR）和鞭毛蛋白 hag 的产生，维持细胞的运动性。我们得出结论，SMB_G1518-1519 蛋白系统在丙酮丁醇梭菌的丁醇敏感性/耐受性表型中发挥作用，可被视为工程化醇耐受性的潜在靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6273/3387226/e1b63ecf5e55/pone.0038815.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6273/3387226/9e0bd524c372/pone.0038815.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6273/3387226/068193bf85b9/pone.0038815.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6273/3387226/347a27c3f19c/pone.0038815.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6273/3387226/8b96fa196769/pone.0038815.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6273/3387226/d0bb74b30a7f/pone.0038815.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6273/3387226/e1b63ecf5e55/pone.0038815.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6273/3387226/9e0bd524c372/pone.0038815.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6273/3387226/068193bf85b9/pone.0038815.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6273/3387226/347a27c3f19c/pone.0038815.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6273/3387226/8b96fa196769/pone.0038815.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6273/3387226/d0bb74b30a7f/pone.0038815.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6273/3387226/e1b63ecf5e55/pone.0038815.g006.jpg

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