Instituto de Química, Universidade Estadual de Campinas, CP 6154, 13083-970 Campinas, SP, Brazil.
Anal Chim Acta. 2012 Jul 29;736:36-44. doi: 10.1016/j.aca.2012.05.037. Epub 2012 May 28.
Mixtures of ethanol, dichloromethane, hexane and acetone obtained according to a statistical design have been used to extract substances from Erythrina speciosa Andrew leaves for chromatographic fingerprinting. The plant extracts from each mixture were analyzed by HPLC-DAD providing UV-vis spectra for each chromatographic peak. These chromatograms and spectra for the design mixtures were then treated with principal component (PCA), Tucker3 and PARAFAC analyses. PCA indicated the existence of five different chromatographic fingerprints for the leave extracts depending on the solvent mixture composition. Different chromatographic peak areas were strongly correlated with the mixture proportions of acetone, dichloromethane and ethanol. Tucker3 and PARAFAC analyses were very useful for identifying simultaneous correlations between chromatographic peak areas, spectral band absorbances and solvent proportions. The acetone proportion was highly correlated with the area of the 3.69 min retention time peak and the spectral absorbances between 250 and 260 nm, consistent with the presence of natural polyphenols. The dichloromethane mixture proportion was strongly correlated with the 12.19 min chromatographic peak area and a single spectral absorbance at 201 nm. This spectral absorption is characteristic of the electronic structures of terpenes and alkaloids.
根据统计设计得到的乙醇、二氯甲烷、正己烷和丙酮混合物已被用于从美丽异木棉叶中提取物质进行色谱指纹分析。每种混合物的植物提取物均通过 HPLC-DAD 进行分析，为每个色谱峰提供紫外可见光谱。然后，对设计混合物的这些色谱图和光谱图进行主成分（PCA）、 Tucker3 和 PARAFAC 分析。PCA 表明，根据溶剂混合物组成，叶提取物存在五种不同的色谱指纹。不同的色谱峰面积与丙酮、二氯甲烷和乙醇的混合物比例强烈相关。Tucker3 和 PARAFAC 分析非常有助于识别色谱峰面积、光谱波段吸光度和溶剂比例之间的同时相关性。丙酮比例与 3.69 分钟保留时间峰的面积和 250 至 260nm 之间的光谱吸光度高度相关，表明存在天然多酚。二氯甲烷混合物比例与 12.19 分钟色谱峰面积和 201nm 处的单一光谱吸光度强烈相关。该光谱吸收是萜类化合物和生物碱电子结构的特征。
