Department of Pathology, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, China.
Tissue Cell. 2012 Dec;44(6):358-64. doi: 10.1016/j.tice.2012.06.001. Epub 2012 Jul 6.
Human mesenchymal stem cells (HMSCs) have been applied in various clinic settings. Ion channels play an important role in cellular physiology. However, the potential role of cationic channels in regulating the proliferation and migration properties of hMSCs remains to be determined. In the present study, the functional expression of ion channels in hMSCs was investigated by patch clamp. MTT assay and BrdU stainings were used to assess the proliferation of hMSCs. hMSC migration was evaluated by Transwell migration assays. The results show that sodium-, L-type calcium, potassium currents have been identified in hMSCs. TEA (K(+) channel blocker), nifedipine (Ca(2+) channel blocker) can inhibit both proliferation and migration of hMSCs. The increase of extracellular Ca(2+) concentration promoted both proliferation and migration of hMSCs. TTX, a Na(+) channel blocker, promoted cell proliferation but inhibited cell migration. Our data suggest that cationic channels (sodium, L-type calcium, potassium channels) play important roles in regulating proliferation and migration of hMSCs.
人骨髓间充质干细胞（HMSCs）已被应用于各种临床环境中。离子通道在细胞生理中起着重要作用。然而，阳离子通道在调节 hMSCs 的增殖和迁移特性方面的潜在作用仍有待确定。在本研究中，通过膜片钳技术研究了 hMSCs 中离子通道的功能表达。MTT 检测和 BrdU 染色用于评估 hMSCs 的增殖。通过 Transwell 迁移实验评估 hMSC 的迁移。结果表明，在 hMSCs 中鉴定出了钠、L 型钙、钾电流。TEA（钾通道阻断剂）、硝苯地平（钙通道阻断剂）可抑制 hMSCs 的增殖和迁移。增加细胞外 Ca2+浓度促进了 hMSCs 的增殖和迁移。TTX，一种钠通道阻断剂，促进细胞增殖但抑制细胞迁移。我们的数据表明，阳离子通道（钠、L 型钙、钾通道）在调节 hMSCs 的增殖和迁移中起着重要作用。
