采用高效液相色谱法分析鱼类中的恶喹酸。
Analysis of oxolinic acid in fish by high-performance liquid chromatography.
作者信息
Björklund H V
机构信息
Institute of Parasitology, Abo Akademi, Finland.
出版信息
J Chromatogr. 1990 Aug 24;530(1):75-82. doi: 10.1016/s0378-4347(00)82304-x.
A simple high-performance liquid chromatographic method for assaying oxolinic acid, a chemotherapeutic agent, in fish tissues has been developed. Nalidixic acid is used as an internal standard. The drugs are separated on an internal surface reversed-phase column. The sample clean-up is minimized. Serum samples are analysed by direct injection on the column; muscle and liver samples are analysed after solid-phase extraction. The recoveries of oxolinic acid from spiked rainbow trout serum, muscle tissue and liver are 99.7, 87.7 and 83.6%, respectively. The lowest measurable amount of the drug is 0.01 microgram/g in all three tissues.
已开发出一种简单的高效液相色谱法，用于测定鱼组织中化疗药物恶喹酸。萘啶酸用作内标。药物在内表面反相柱上分离。样品净化降至最低限度。血清样品通过直接进样到柱上进行分析；肌肉和肝脏样品在固相萃取后进行分析。恶喹酸在加标虹鳟鱼血清、肌肉组织和肝脏中的回收率分别为99.7%、87.7%和83.6%。在所有三种组织中，该药物的最低可测量量为0.01微克/克。
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