Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, Tamil Nadu 608002, India.
Eur J Pharmacol. 2012 Sep 15;691(1-3):143-50. doi: 10.1016/j.ejphar.2012.06.038. Epub 2012 Jul 4.
The present study was designed to investigate the beneficial role of diosgenin on oxidative stress markers and histopathological changes in aorta of streptozotocin induced diabetic rats. Diabetes was induced in experimental rats by a single intraperitoneal injection of streptozotocin (55 mg/kg body weight (b.w.)). From the sixth week, experimental rats received diosgenin at different doses (10, 20 and 40 mg/kg b.w.) once daily for 4 weeks. At the end of the experimental periods, diabetic rats exhibited significant increase in the levels of plasma glucose, glycosylated hemoglobin with significant decrease in insulin and total hemoglobin. The activities of antioxidant enzymes such as superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and the levels of reduced glutathione were decreased while increases in the levels of lipid peroxidation markers were observed in aortic tissues of diabetic rats. Oral administration of diosgenin to diabetic rats significantly decreased the plasma glucose and increased the insulin level based on a dose dependent manner. Diosgenin at a dose of 40 mg/kg b.w. was more pronounced effect than the other two doses and used for further studies. All the manifestations observed in diabetic rats were significantly reversed to near normal at a dose of 40 mg/kg b.w. of diosgenin. These findings suggest that diosgenin could have a beneficial role against aortic damage induced by oxidative stress in diabetic state, which was evidenced by the propensity of diosgenin to modulate the antioxidant defense and to decrease the lipid peroxidation in aorta.
本研究旨在探讨薯蓣皂素对链脲佐菌素诱导的糖尿病大鼠氧化应激标志物和主动脉组织病理学变化的有益作用。实验大鼠通过单次腹腔注射链脲佐菌素（55mg/kg 体重（b.w.））诱导糖尿病。从第 6 周开始，实验大鼠每天接受不同剂量（10、20 和 40mg/kg b.w.）的薯蓣皂素治疗，共 4 周。在实验期末，糖尿病大鼠的血浆葡萄糖、糖化血红蛋白水平显著升高，胰岛素和总血红蛋白水平显著降低。糖尿病大鼠主动脉组织中抗氧化酶如超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽-S-转移酶的活性降低，脂质过氧化标志物的水平升高。薯蓣皂素口服给予糖尿病大鼠可显著降低血糖，并呈剂量依赖性增加胰岛素水平。薯蓣皂素 40mg/kg b.w.的剂量比其他两个剂量更显著，因此用于进一步研究。薯蓣皂素 40mg/kg b.w.的剂量可显著逆转糖尿病大鼠的所有表现，使其接近正常水平。这些发现表明，薯蓣皂素可能对糖尿病状态下氧化应激引起的主动脉损伤具有有益作用，这可通过薯蓣皂素调节抗氧化防御和降低主动脉脂质过氧化作用来证明。
