Department of Cancer Research, Key Laboratory of Molecular Microbiology and Technology of Ministry of Education, College of Life Sciences, Nankai University, PR China.
FEBS Lett. 2012 Sep 21;586(19):3215-21. doi: 10.1016/j.febslet.2012.06.039. Epub 2012 Jul 3.
The significance of hepatitis B virus (HBV) DNA-based integration in hepatocarcinogenesis is poorly understood. In the present study, we investigated whether the integration of HBV X gene (HBx) is involved in the event. Our finding showed that the integration of HBx fragment (316-462 bp/262-462 bp) was able to transform human immortalized normal liver LO2 cells using a cell model of HBx-integration. We identified that the recombination, HBx/Alu core sequence/subtelomeric DNA, was required for the transformation, which could be detected in 5 out of 44 clinical HBx-positive hepatocellular carcinoma tissues. Thus, we conclude that HBx integration is involved in the hepatocarcinogenesis.
乙型肝炎病毒 (HBV) DNA 整合在肝癌发生中的意义尚未完全明确。本研究旨在探讨 HBV X 基因 (HBx) 的整合是否参与了这一事件。我们的研究结果表明，使用 HBx 整合细胞模型，能够将 HBx 片段（316-462bp/262-462bp）整合到永生化正常人类肝脏 LO2 细胞中，导致其转化。我们鉴定出重组、HBx/Alu 核心序列/端粒外 DNA 是转化所必需的，在 44 例 HBx 阳性肝细胞癌组织中有 5 例可检测到这种重组。因此，我们得出结论，HBx 整合参与了肝癌的发生。
