Department of Endocrinology, The Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, China.
Biochem Biophys Res Commun. 2012 Aug 3;424(3):462-8. doi: 10.1016/j.bbrc.2012.06.133. Epub 2012 Jul 4.
Adipose tissue is now recognized to be an important endocrine organ, secreting a variety of adipokines that are involved in the regulation of energy metabolism, insulin resistance and metabolic syndrome. C-reactive protein (CRP) is considered as one of the most sensitive markers of inflammation. A number of studies have shown that elevation of CRP concentrations is an independent predictive parameter of type 2 diabetes mellitus, which is also strongly associated with various components of the metabolic syndrome. The aim of the present study is to investigate the effects of CRP on adipokines genes expression in 3T3-L1 adipocytes. Quantitative real-time PCR analysis revealed that CRP inhibited adiponectin, leptin and peroxisome proliferator-activated receptor-gamma (PPAR-γ) genes expression and raised tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) mRNA levels in matured 3T3-L1 adipocytes in a dose and time-dependent manner. Pharmacological inhibition of phosphatidylinositol (PI)-3 kinase by wortmannin partially reversed the effects of CRP on adiponectin, TNF-α and leptin genes expression. These results collectively suggest that CRP regulates adiponectin, TNF-α, leptin, IL-6 and PPAR-γ genes expression, and that might represent a mechanism by which CRP regulates insulin resistance, obesity and metabolic syndrome.
脂肪组织现在被认为是一个重要的内分泌器官，分泌各种脂肪因子，参与能量代谢、胰岛素抵抗和代谢综合征的调节。C 反应蛋白（CRP）被认为是炎症最敏感的标志物之一。许多研究表明，CRP 浓度的升高是 2 型糖尿病的独立预测参数，它也与代谢综合征的各种成分密切相关。本研究旨在探讨 CRP 对 3T3-L1 脂肪细胞脂肪因子基因表达的影响。定量实时 PCR 分析显示，CRP 以剂量和时间依赖的方式抑制成熟 3T3-L1 脂肪细胞中脂联素、瘦素和过氧化物酶体增殖物激活受体-γ（PPAR-γ）基因的表达，并升高肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）mRNA 水平。PI-3 激酶的药理学抑制剂wortmannin 部分逆转了 CRP 对脂联素、TNF-α 和瘦素基因表达的影响。这些结果表明，CRP 调节脂联素、TNF-α、瘦素、IL-6 和 PPAR-γ 基因的表达，这可能代表了 CRP 调节胰岛素抵抗、肥胖和代谢综合征的一种机制。
