Zhou Hui, Liu Jinkang, Chen Shengxi, Xiong Zeng, Zhou Jianhua, Tong Shiyu, Chen Hao, Zhou Moling
Department of Radiology, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2012 Jun;37(6):555-60. doi: 10.3969/j.issn.1672-7347.2012.06.003.
To explore the degree, mechanism and clinical significance of three-dimensional tumor microvascular architecture phenotype heterogeneity (3D-TMAPH) in non-small cell carcinoma (NSCLC).
Twenty-one samples of solitary pulmonary nodules were collected integrally. To establish two-dimensional tumor microvascular architecture phenotype (2D-TMAP) and three-dimensional tumor microvascular architecture phenotype (3D-TMAP), five layers of each nodule were selected and embedded in paraffin. Test indices included the expressions of vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA), EphB4, ephfinB2 and microvascular density marked by anti-CD34 (CD34-MVD). The degrees of 3D-TMAPH were evaluated by the coefficient of variation and extend of heterogeneity. Spearman rank correlation analysis was used to investigate the relationships between 2D-TMAP, 3D-TMAP and clinicopathological features.
3D-TMAPH showed that 2D-TMAP heterogeneity was expressed in the tissues of NSCLC. The heterogeneities in the malignant nodules were significantly higher than those in the active inflammatory nodules and tubercular nodules. In addition, different degrees of heterogeneity of CD34-MVD and PCNA were found in NSCLC tissues. The coefficients of variation of CD34- MVD and PCNA were positively related to the degree of differentiation (all P<0.05), but not related to the P-TNM stages, histological type or lymphatic metastasis (all P>0.05). The level of heterogeneity of various expression indexes (ephrinB2, EphB4, VEGF) in NSCLC tissues were inconsistent, but there were no significant differences in heterogeneity in NSCLC tissues with different histological types (P>0.05).
3D-TMAPH exists widely in the microenvironment during the genesis and development of NSCLC and has a significant impact on its biological complexity.
探讨非小细胞肺癌（NSCLC）中三维肿瘤微血管构筑表型异质性（3D-TMAPH）的程度、机制及临床意义。
完整收集21例孤立性肺结节样本。为建立二维肿瘤微血管构筑表型（2D-TMAP）和三维肿瘤微血管构筑表型（3D-TMAP），选取每个结节的五层组织并石蜡包埋。检测指标包括血管内皮生长因子（VEGF）、增殖细胞核抗原（PCNA）、EphB4、ephrinB2的表达以及抗CD34标记的微血管密度（CD34-MVD）。通过变异系数和异质性范围评估3D-TMAPH的程度。采用Spearman等级相关分析研究2D-TMAP、3D-TMAP与临床病理特征之间的关系。
3D-TMAPH显示NSCLC组织中存在2D-TMAP异质性。恶性结节中的异质性显著高于活动性炎性结节和结核结节。此外，在NSCLC组织中发现了不同程度的CD34-MVD和PCNA异质性。CD34-MVD和PCNA的变异系数与分化程度呈正相关（均P<0.05），但与P-TNM分期、组织学类型或淋巴转移无关（均P>0.05）。NSCLC组织中各种表达指标（ephrinB2、EphB4、VEGF）的异质性水平不一致，但不同组织学类型的NSCLC组织中异质性无显著差异（P>0.05）。
3D-TMAPH在NSCLC发生发展过程的微环境中广泛存在，对其生物学复杂性有显著影响。
