College of Biological Sciences, China Agricultural University, Beijing, China.
Nat Methods. 2012 Sep;9(9):904-6. doi: 10.1038/nmeth.2099. Epub 2012 Jul 8.
We have developed pLink, software for data analysis of cross-linked proteins coupled with mass-spectrometry analysis. pLink reliably estimates false discovery rate in cross-link identification and is compatible with multiple homo- or hetero-bifunctional cross-linkers. We validated the program with proteins of known structures, and we further tested it on protein complexes, crude immunoprecipitates and whole-cell lysates. We show that it is a robust tool for protein-structure and protein-protein-interaction studies.
我们开发了 pLink 软件，用于分析与质谱分析相结合的交联蛋白质数据。pLink 能够可靠地估计交联鉴定中的错误发现率，并且与多种同型或异型双功能交联剂兼容。我们使用已知结构的蛋白质对该程序进行了验证，并且进一步在蛋白质复合物、粗免疫沉淀物和全细胞裂解物上对其进行了测试。我们表明，它是用于蛋白质结构和蛋白质-蛋白质相互作用研究的强大工具。
