State Key Laboratory of Cancer Biology, Department of Gastrointestinal Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, 710032, Shanxi, People's Republic of China.
Med Oncol. 2012 Dec;29(5):3136-42. doi: 10.1007/s12032-012-0284-y. Epub 2012 Jul 8.
We had reported that MSP58 regulates colorectal cancer cell proliferation, development, and apoptosis, by the cyclin D1-cyclin-dependent kinase 4-p21 pathway. In this study, MSP58 protein expression was examined by immunohistochemistry in 499 specimens of CRC. The relationship between various clinicopathological features and overall patient survival rate was analyzed. The association of MSP58 expression with the 499 CRC patients' survival rate was assessed by Kaplan-Meier and Cox regression. Using ROC curve to provide sensitivity and specificity of the score of MSP58 predicts local recurrence and survival of CRC patients. The expression of MSP58 was positively correlated with the depth of invasion (P < 0.001), local recurrence (P = 0.008), tumor grade (P = 0.002), and UICC stage (P < 0.001). The Kaplan-Meier survival analysis demonstrated that the survival time of CRC patients with low expression of MSP58 was longer than those with high expression during the 5-year follow-up period (P < 0.001). COX regression analysis indicated that high expression of MSP58 (P < 0.001), depth of invasion >pT(1) (P = 0.008), distant organ metastasis (pM(1)) (P < 0.001), regional lymph node metastasis (≥ pN(1)) (P < 0.001), and local recurrence (Yes) (P = 0.007) were independent, poor prognostic factors of CRC. ROC curve showed the score of MSP58 expression level did provide a maximal sensitivity and specificity to predict local recurrence and survival of CRC patients. Our results demonstrated MSP58 might serve as a novel prognostic marker that is independent of, and additive to, the UICC staging system.
我们曾报道 MSP58 通过 cyclin D1-cyclin 依赖性激酶 4-p21 通路调节结直肠癌细胞的增殖、发育和凋亡。本研究采用免疫组织化学方法检测了 499 例 CRC 标本中的 MSP58 蛋白表达情况。分析了各种临床病理特征与总患者生存率之间的关系。通过 Kaplan-Meier 和 Cox 回归分析评估 MSP58 表达与 499 例 CRC 患者生存率的关系。使用 ROC 曲线提供 MSP58 评分预测 CRC 患者局部复发和生存的敏感性和特异性。MSP58 的表达与浸润深度（P < 0.001）、局部复发（P = 0.008）、肿瘤分级（P = 0.002）和 UICC 分期（P < 0.001）呈正相关。Kaplan-Meier 生存分析表明，在 5 年随访期间，MSP58 低表达的 CRC 患者的生存时间长于高表达的患者（P < 0.001）。COX 回归分析表明，MSP58 高表达（P < 0.001）、浸润深度>TpT（1）（P = 0.008）、远处器官转移（pM（1））（P < 0.001）、区域淋巴结转移（≥pN（1））（P < 0.001）和局部复发（是）（P = 0.007）是 CRC 的独立、不良预后因素。ROC 曲线表明，MSP58 表达水平评分可提供最大的敏感性和特异性，以预测 CRC 患者的局部复发和生存。我们的结果表明，MSP58 可能作为一个新的独立于 UICC 分期系统的预后标志物。
