Dyachuk V, Wanninger A, Voronezhskaya Elena E
A. V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch of Russian Academy of Sciences, 690059 Vladivostok, Russia.
Acta Biol Hung. 2012;63 Suppl 2:221-9. doi: 10.1556/ABiol.63.2012.Suppl.2.30.
Bivalve larvae use catch muscles for rapid shell closure and maintenance of the closed condition. We used specific antibodies against the muscle proteins together with phalloidin and neuronal markers, FMRFamide and serotonin (5-HT), to analyze mutual distribution of muscle and neuronal elements in larvae of the mussel, Mytilus trossulus, and the oyster, Crassostrea gigas. At trochophore and early veliger stages no anatomical connections between muscular and nervous system were detected. By the pediveliger stage the 5-HT innervation of the anterior adductor developed in oyster only, while rich FMRFa innervation of the adductor muscles developed in both species. Possible roles and mechanisms of FMRFamide and serotonin in the regulation of the catch state are discussed.
双壳类幼虫利用捕捉肌实现贝壳的快速闭合以及维持闭合状态。我们使用针对肌肉蛋白的特异性抗体，结合鬼笔环肽以及神经标记物FMRF酰胺和5-羟色胺（5-HT），来分析贻贝（Mytilus trossulus）和牡蛎（Crassostrea gigas）幼虫中肌肉和神经成分的相互分布。在担轮幼虫和早期面盘幼虫阶段，未检测到肌肉系统和神经系统之间的解剖学联系。到足面盘幼虫阶段，仅在牡蛎中观察到前闭壳肌有5-HT神经支配，而在这两个物种中闭壳肌均有丰富的FMRFa神经支配。文中讨论了FMRF酰胺和5-羟色胺在调节捕捉状态中的可能作用和机制。
