细胞周期蛋白D1在人垂体肿瘤中的差异表达：与MIB-1和p27/Kip1标记指数的关系。

Differential expression of cyclin D1 in human pituitary tumors: relation to MIB-1 and p27/Kip1 labeling indices.

作者信息

Hewedi Iman H, Osman Wesam M, El Mahdy Manal M

机构信息

Department of Pathology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

出版信息

J Egypt Natl Canc Inst. 2011 Dec;23(4):171-9. doi: 10.1016/j.jnci.2011.11.003. Epub 2011 Dec 29.

DOI:10.1016/j.jnci.2011.11.003

PMID:22776845

Abstract

BACKGROUND

Pituitary tumors are a common form of endocrine neoplasia. However few studies assessed the expression of the principal cyclin regulating checkpoint exit, cyclin D1. Cyclin D1 expression in pituitary tumors and its possible relation to MIB-1 and p27/Kip1 labeling indices (LIs) was explored.

DESIGN

We studied a total of 199 pituitaries, including normal pituitaries (n=7), pituitary adenomas (n=187), and pituitary carcinoma (n=5). All tissues were tested as cores of archived tissue microarrays that were immunostained for cyclin D1, MIB-1 and p27 using a standard technique. Tissue cores were subjected to automated analysis to evaluate the staining LIs.

RESULTS

No cyclin D1 positive cells in the normal anterior pituitary gland was found. Sparse nuclear staining was noted in pituitary tumors. Higher expression of cyclin D1 was noted in pituitary carcinomas compared to adenomas (p<0.001), in non-functioning adenomas compared to functioning ones (p<0.001) in macroadenomas versus microadenomas (p=0.017) and in recurrent non recurrent adenomas (p<0.001). Cyclin D1 LI and MIB-1 LI were related among adenomas (p<0.001) and carcinomas (p=0.041). p27 LI was neither related to pituitary adenoma recurrence nor invasion.

CONCLUSIONS

Expression of cyclin D1 in pituitary tumors is related to cell proliferation, recurrence, and metastatic potential. Nuclear cyclin D1 expression is a good marker of aggressive behavior in pituitary tumors.

摘要

背景

垂体肿瘤是内分泌肿瘤的常见形式。然而，很少有研究评估调节检查点退出的主要细胞周期蛋白细胞周期蛋白D1的表达。本研究探讨了垂体肿瘤中细胞周期蛋白D1的表达及其与MIB-1和p27/Kip1标记指数（LIs）的可能关系。

设计

我们共研究了199个垂体，包括正常垂体（n = 7）、垂体腺瘤（n = 187）和垂体癌（n = 5）。所有组织均作为存档组织微阵列的芯进行检测，采用标准技术对细胞周期蛋白D1、MIB-1和p27进行免疫染色。对组织芯进行自动分析以评估染色LIs。

结果

在正常垂体前叶未发现细胞周期蛋白D1阳性细胞。在垂体肿瘤中观察到稀疏的核染色。与腺瘤相比，垂体癌中细胞周期蛋白D1的表达更高（p < 0.001），与功能性腺瘤相比，无功能性腺瘤中细胞周期蛋白D1的表达更高（p < 0.001），大腺瘤与微腺瘤相比（p = 0.017），复发性与非复发性腺瘤相比（p < 0.001）。腺瘤（p < 0.001）和癌（p = 0.041）中细胞周期蛋白D1 LI和MIB-1 LI相关。p27 LI与垂体腺瘤的复发和侵袭均无关。