College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China.
Luminescence. 2013 May-Jun;28(3):368-71. doi: 10.1002/bio.2391. Epub 2012 Jul 9.
Photobacteria were used as test objects for rapid monitoring of ecotoxicants. Specific inhibitory effects of Hg(2+) on bioluminescence and cell growth as well as the lux gene expression of Photobacterium leiognathi were studied. The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction assay was used to evaluate cellular proliferation and mortality. The luminescent inhibition effect on P. leiognathi cells was found to increase as cellular mortality increased; y = 0.744x - 4.8916, R² = 0.9794. However, this trend was not observed in cell growth processes. Quantitation of lux mRNAs by semi-quantitative RT-qPCR indicated that increases and decreases in luciferase mRNA integral level coincided with changes in luminescence intensity (R(2) = 0.93). Addition of Hg(2+) changed luminescence but without concomitant changes in extractable luciferase activity. Nevertheless, the presence of Hg(2+) changed lux gene expression. This phenomenon requires further research.
采用发光细菌作为测试对象，对环境毒物进行快速监测。本文以发光杆菌为指示生物，研究了 Hg(2+)对发光强度、细胞生长及 lux 基因表达的特异性抑制效应。采用 MTT 比色法检测细胞的增殖和死亡率。结果表明，Hg(2+)对发光细菌的抑制作用随细胞死亡率的增加而增强，y = 0.744x - 4.8916，R² = 0.9794。然而，在细胞生长过程中并未观察到这种趋势。半定量 RT-qPCR 定量 lux mRNAs 的结果表明，荧光素酶 mRNA 整体水平的增加和减少与发光强度的变化一致(R(2) = 0.93)。Hg(2+)的加入虽然改变了发光强度，但并未改变可提取的荧光素酶活性。然而，lux 基因的表达确实发生了变化。这一现象需要进一步研究。
