Basha Mahaboob, Poojary Annappa
Department of Zoology, Bangalore University, Bangalore, Karnataka, India.
Toxicol Int. 2012 May;19(2):173-81. doi: 10.4103/0971-6580.97219.
The adverse effects produced by chlorpyrifos (CPF) or cold stress alone in humans and animals are well documented, but there is no information available relating to the consequences of their co- exposure in an age-related manner. In this study, effects of sublethal doses of CPF were carried out in vivo, for 48 h to assess the biochemical perturbations in relation to interactions with cold stress (15°C and 20°C) in different age group rat CNS. A positive interaction of CPF with age of animal and cold exposure was observed resulting in marked decrease in the activity levels of AChE (P<0.05), ChAT (P<0.05), Na(+), K(+)-ATPase (P<0.05), Ca(2+)-ATPase (P<0.05), and Mg(2+)-ATPase (P<0.05). The ANOVA and posthoc analysis showed that regulatory enzymes decreased significantly (P<0.05) on CPF exposure. Overall, the effect of co-exposure was appreciably different from either of the exposures. Synergistic interaction of CPF and cold stress at 15°C showed higher inhibition in comparison with CPF and cold stress alone and together at 20°C. Further, this study reveals that young animals are significantly vulnerable and sensitive than adults.
毒死蜱（CPF）或单独的冷应激对人和动物产生的不良影响已有充分记录，但尚无关于它们以年龄相关方式共同暴露后果的信息。在本研究中，对不同年龄组大鼠中枢神经系统进行了48小时的体内亚致死剂量CPF作用实验，以评估与冷应激（15°C和20°C）相互作用相关的生化扰动。观察到CPF与动物年龄和冷暴露之间存在正相互作用，导致乙酰胆碱酯酶（AChE）（P<0.05）、胆碱乙酰转移酶（ChAT）（P<0.05）、钠钾ATP酶（P<0.05）、钙ATP酶（P<0.05）和镁ATP酶（P<0.05）的活性水平显著降低。方差分析和事后分析表明，CPF暴露后调节酶显著降低（P<0.05）。总体而言，共同暴露的影响与单独的任何一种暴露明显不同。与单独的CPF和20°C的冷应激以及两者一起相比，15°C时CPF与冷应激的协同相互作用表现出更高的抑制作用。此外，本研究表明幼龄动物比成年动物明显更易受影响且更敏感。
