[去甲肾上腺素和乙酰胆碱对大鼠下丘脑切片室旁核神经元电活动的影响]

[Effects of noradrenaline and acetylcholine on electric activity of paraventricular neurons of rat hypothalamic slices].

作者信息

Xing B R, Guo Y M, Xia J H, Chen Y Z

机构信息

Department of Physiology, Second Military Medical College, Shanghai.

出版信息

Sheng Li Xue Bao. 1990 Oct;42(5):413-9.

PMID:2278003

Abstract

The effects of noradrenaline (NA) and acetylcholine (ACh) on 90 paraventricular neurons from 31 hypothalamic slices prepared from rat were observed. After perfusing the brain slices with NA (10(-6) mol/L, 3 min), discharge rate of 14/73 (17%) nonphasic and 7/12 (58.3%) phasic neurons was significantly increased, while that of 10/73 (12%) nonphasic and 2/12 (16.6%) phasic neurons was significantly decreased, even ceased. 50/73 (64%) of the nonphasic and 3/12 (25%) of the phasic neurons were non-responsive. The effects of NA on the nonphasic neurons were completely blocked by alpha receptor blocker phentolamine. The effects of NA on phasic neurons were partly blocked by phentolamine or propranolol (beta receptor blocker). After perfusing hypothalamic slices with ACh (10(-7) mol/L, 3 min), the firing rate of 15/73 (19%) nonphasic and 6/12 (50%) phasic neurons was increased, while that of 9/73 (11%) nonphasic and 2/12 (16.6%) phasic neurons was decreased. 54/73 (69%) nonphasic and 4/12 (33.3%) phasic neurons were non-responsive. The effect of ACh on the neurons could be reversed by atropine. The effect of scopolamine (M blocker) is similar to that of atropine. The experimental results suggest that the excitatory or inhibitory effect of NA or ACh on paraventricular neurons has mediated respectively by alpha, beta or M receptors.

摘要

观察了去甲肾上腺素（NA）和乙酰胆碱（ACh）对31只大鼠下丘脑切片中90个室旁核神经元的作用。用NA（10⁻⁶mol/L，3分钟）灌注脑片后，14/73（17%）的非位相性神经元和7/12（58.3%）的位相性神经元放电频率显著增加，而10/73（12%）的非位相性神经元和2/12（16.6%）的位相性神经元放电频率显著降低，甚至停止放电。50/73（64%）的非位相性神经元和3/12（25%）的位相性神经元无反应。NA对非位相性神经元的作用被α受体阻滞剂酚妥拉明完全阻断。NA对位相性神经元的作用被酚妥拉明或普萘洛尔（β受体阻滞剂）部分阻断。用ACh（10⁻⁷mol/L，3分钟）灌注下丘脑切片后，15/73（19%）的非位相性神经元和6/12（50%）的位相性神经元放电频率增加，而9/73（11%）的非位相性神经元和2/12（16.6%）的位相性神经元放电频率降低。54/73（69%）的非位相性神经元和4/12（33.3%）的位相性神经元无反应。ACh对神经元的作用可被阿托品逆转。东莨菪碱（M受体阻滞剂）的作用与阿托品相似。实验结果表明，NA或ACh对室旁核神经元的兴奋或抑制作用分别由α、β或M受体介导。