Min Min, Lin Li, Bi Cheng-Feng, Wang Xiao-Qing, Luo Tian-You, Zhao Sha, Zhang Wen-Yan, Liu Wei-Ping
Department of Pathology, West China Hospital of Sichuan University, Chengdu 610041, China.
Zhonghua Zhong Liu Za Zhi. 2012 Feb;34(2):110-6. doi: 10.3760/cma.j.issn.0253-3766.2012.02.007.
To analyze the subtypes of primary diffuse large B cell lymphoma of the central nervous system (CNS DLBCL) and to explore the relationship between the subtype classification and prognosis.
Immunohistochemical staining was used to determine the expression of CD20, CD3, CD10, Bcl-6, Mum-1, CD5, Bcl-2, Ki-67, FOXP-1, GCET-1, BLIMP-1 and LMO-2 antigens on paraffin-embedded sections of 47 cases. Hans, Choi and Tally subtypes were classified, and univariate and multivariate analyses were used to elucidate the relationship between the subtypes and prognosis.
In the 47 cases, the expression of Bcl-2 in the tumor cells was 46.8%, CD10 4.3%, Bcl-6 70.2%, Mum-1 53.2%, GCET-1 36.2%, BLIMP-1 4.3%, FOXP-1 63.8% and LMO-2 19.2%. The positive rate of Ki-67 was 30% to 95%, with a median of 80%, of which 12 cases (25.5%) was > or = 90%. The Hans subtype classification showed 16 cases (34.0%) were of GCB type and 31 cases (66.0%) of non-GCB type. The Choi subtype classification showed 16 cases (34.0%) were of GCB type and 31 cases (66.0%) of ABC type. The Tally subtype classification showed 6 cases (12.8%) were of GCB type and 41 cases (87.2%) of non-GCB type.
The results of this study show that there is no significant correlation between the three subtypes and prognosis. The prognosis is correated with post-operative radiotherapy, chemotherapy and MTX therapy.
分析原发性中枢神经系统弥漫性大B细胞淋巴瘤（CNS DLBCL）的亚型，并探讨亚型分类与预后之间的关系。
采用免疫组织化学染色法检测47例石蜡包埋切片中CD20、CD3、CD10、Bcl-6、Mum-1、CD5、Bcl-2、Ki-67、FOXP-1、GCET-1、BLIMP-1和LMO-2抗原的表达。进行Hans、Choi和Tally亚型分类，并采用单因素和多因素分析阐明亚型与预后之间的关系。
47例中，肿瘤细胞中Bcl-2的表达率为46.8%，CD10为4.3%，Bcl-6为70.2%，Mum-1为53.2%，GCET-1为36.2%，BLIMP-1为4.3%，FOXP-1为63.8%，LMO-2为19.2%。Ki-67阳性率为30%至95%，中位数为80%，其中12例（25.5%）≥90%。Hans亚型分类显示，16例（34.0%）为生发中心B细胞（GCB）型，31例（66.0%）为非GCB型。Choi亚型分类显示，16例（34.0%）为GCB型，31例（66.0%）为活化B细胞（ABC）型。Tally亚型分类显示，6例（1２.8%）为GCB型，41例（87.2%）为非GCB型。
本研究结果表明，三种亚型与预后之间无显著相关性。预后与术后放疗、化疗及甲氨蝶呤治疗相关。
