Murugova Tatiana N, Klacsová Mária, Pullmannová Petra, Karlovská Janka, Balgavý Pavol
Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, Dubna, Russia.
Gen Physiol Biophys. 2012 Jun;31(2):225-7. doi: 10.4149/gpb_2012_021.
The excimer 1,2-dipyrenedecanoyl-sn-glycero-3-phosphatidylcholine (dipy10PC) fluorescence probe was used to determine effects of aliphatic alcohols (CnH2n+1OH, n = 12-18 is the even number of carbons in alkyl chain) on fluid dioleoylphosphatidylcholine (DOPC) +dioleoylphosphatidylserine (DOPS) bilayers in multilamellar vesicles at molar ratio DOPC/DOPS = 24.7. The excimer to monomer fluorescence intensity ratio increases with the increase of CnH2n+1OH/DOPC molar ratio and decreases with the CnH2n+1OH alkyl chain length n at a constant CnH2n+1OH/DOPC = 0.4 molar ratio. These effects indicate changes in the bilayer lateral pressure on the level of pyrenyl moieties location.
准分子1,2 - 二芘癸酰 - sn - 甘油 - 3 - 磷脂酰胆碱（dipy10PC）荧光探针用于测定脂肪醇（CnH2n + 1OH，n = 12 - 18为烷基链中碳原子数为偶数）对多层囊泡中流体二油酰磷脂酰胆碱（DOPC）+二油酰磷脂酰丝氨酸（DOPS）双层膜的影响，其中DOPC/DOPS摩尔比为24.7。在恒定的CnH2n + 1OH/DOPC = 0.4摩尔比下，准分子与单体荧光强度比随CnH2n + 1OH/DOPC摩尔比的增加而增加，随CnH2n + 1OH烷基链长度n的增加而降低。这些影响表明芘基部分位置水平上双层膜侧向压力的变化。
