Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, PR China.
Pancreas. 2012 Aug;41(6):845-8. doi: 10.1097/MPA.0b013e318247d784.
The aim of the present study was to develop a model of hypertriglyceridemic (HTG) acute pancreatitis and to investigate the effects of probucol in this model.
Hamsters were fed a high-fat diet (HFD) or a normal diet for 3 weeks. Probucol was added at 1% to the HFD in the treated group. Pancreatitis was induced by 7 peritoneal injections of cerulein to the normal and HFD hamster groups. The severity of the pancreatitis and whole body oxidative stress were assessed.
The HFD induced severe HTG (>1000 mg/dL) in the hamsters. A more severe pancreatitis was observed in the HFD group. The HFD did not influence plasma-reduced glutathione level, but there was a significant increase after 1% probucol was provided in the diet. Plasma malonaldehyde levels in the HFD group were significantly higher than the normal chow group, whereas probucol administration significantly decreased plasma hydrogen peroxide and malonaldehyde levels. We also found that probucol significantly reduced levels of amylase and lipase in the plasma and pathological scores in pancreatic tissue.
This study presents a novel model of severe HTG acute pancreatitis, and our results support the potential therapeutic application of probucol in HTG acute pancreatitis.
本研究旨在建立一种高甘油三酯血症（HTG）性急性胰腺炎模型，并探讨普罗布考在此模型中的作用。
给予仓鼠高脂饮食（HFD）或正常饮食 3 周。在治疗组中，将 1%的普罗布考添加到 HFD 中。用 7 次腹腔注射促胰液素诱导正常和 HFD 仓鼠组的胰腺炎。评估胰腺炎的严重程度和全身氧化应激。
HFD 诱导仓鼠出现严重的 HTG（>1000mg/dL）。HFD 组观察到更严重的胰腺炎。HFD 不影响血浆还原型谷胱甘肽水平，但在饮食中添加 1%普罗布考后，其水平显著升高。HFD 组血浆丙二醛水平明显高于正常饲料组，而普罗布考给药可显著降低血浆过氧化氢和丙二醛水平。我们还发现，普罗布考可显著降低血浆中淀粉酶和脂肪酶水平以及胰腺组织的病理评分。
本研究提出了一种新的严重 HTG 性急性胰腺炎模型，我们的结果支持普罗布考在 HTG 性急性胰腺炎中的潜在治疗应用。
