Jensen Jørgen Skov
Statens Serum Institut, Copenhagen, Denmark.
Methods Mol Biol. 2012;903:129-39. doi: 10.1007/978-1-61779-937-2_8.
Mycoplasma genitalium is an established cause of male nongonococcal urethritis, in particular in cases with recurrent disease and in those negative for Chlamydia trachomatis. In women M. genitalium causes cervicitis and there is increasing evidence that it is causing pelvic inflammatory disease (PID). Nucleic acid amplification tests are currently the only available methods for detection, but no commercially available tests have been thoroughly evaluated. Here we describe a TaqMan-based PCR test for detection of the infection and a conventional PCR that serves as a confirmatory assay with the possibility of sequencing the product for detection of macrolide resistance-mediating mutations.
生殖支原体是男性非淋菌性尿道炎的确切病因，尤其是在复发性疾病以及沙眼衣原体检测呈阴性的病例中。在女性中，生殖支原体可引起宫颈炎，并且越来越多的证据表明它会导致盆腔炎（PID）。核酸扩增检测是目前唯一可用的检测方法，但尚未对任何市售检测方法进行全面评估。在此，我们描述了一种基于TaqMan的PCR检测方法用于检测该感染，以及一种传统PCR方法作为确证检测，有可能对产物进行测序以检测介导大环内酯类耐药性的突变。
