用于乙型肝炎病毒感染中病毒载量测定的基于延伸的实时荧光定量PCR光使用方案。
Protocol for the use of light upon extension real-time PCR for the determination of viral load in HBV infection.
作者信息
Li Guimin, Li Wangfeng, Liu Lixia
机构信息
School of Life Sciences, Northeast Normal University, Changchun, People's Republic of China.
出版信息
Methods Mol Biol. 2012;903:273-82. doi: 10.1007/978-1-61779-937-2_18.
Real-time PCR has engendered wide acceptance for quantitation of hepatitis B virus (HBV) DNA in the blood due to its improved rapidity, sensitivity, reproducibility, and reduced contamination. Here we describe a cost-effective and highly sensitive HBV real-time quantitative assay based on the light upon extension real-time PCR platform and a simple and reliable HBV DNA preparation method using silica-coated magnetic beads.
由于实时荧光定量聚合酶链反应（Real-time PCR）具有更高的速度、灵敏度、可重复性以及更低的污染风险，它已被广泛用于定量检测血液中的乙型肝炎病毒（HBV）DNA。在此，我们描述了一种基于延伸发光实时荧光定量聚合酶链反应平台的经济高效且高灵敏度的HBV实时定量检测方法，以及一种使用二氧化硅包被磁珠的简单可靠的HBV DNA制备方法。
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