水溶性1-乙基-3-(3-二甲氨基丙基)碳二亚胺在荧光法测定糖醛酸和羧酸中的应用。

Use of water-soluble 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide for the fluorescent determination of uronic acids and carboxylic acids.

作者信息

Kobayashi M, Ichishima E

机构信息

Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University, Miyagi, Japan.

出版信息

Anal Biochem. 1990 Aug 15;189(1):122-5. doi: 10.1016/0003-2697(90)90056-f.

DOI:10.1016/0003-2697(90)90056-f

PMID:2278381

Abstract

Reaction between glucuronic acid and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) was monitored by the o-phthalaldehyde (OPA) method, which was developed for the fluorescent assay of compounds containing an amino group. About 1 nmol of glucuronic acid was detected by this method. This EDC-OPA method was effective in detecting not only acidic sugar but also carboxylic acid. Although the sensitivity of the EDC-OPA method was somewhat lower than that of amino acid determination by OPA, a very simple and convenient assay was attained for compounds containing a carboxyl group.

摘要

通过邻苯二甲醛（OPA）法监测葡萄糖醛酸与1-乙基-3-（3-二甲基氨基丙基）碳二亚胺（EDC）之间的反应，该方法是为含氨基化合物的荧光测定而开发的。用该方法可检测到约1 nmol的葡萄糖醛酸。这种EDC-OPA方法不仅对酸性糖有效，对羧酸也有效。虽然EDC-OPA方法的灵敏度略低于OPA测定氨基酸的灵敏度，但对于含羧基的化合物而言，它是一种非常简单便捷的检测方法。