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焚烧乳牙的宏观和微观变化。

Macroscopic and microscopic changes in incinerated deciduous teeth.

作者信息

Karkhanis S, Ball J, Franklin D

机构信息

Centre for Forensic Science, The University of Western Australia, Crawley, Western Australia-6009.

出版信息

J Forensic Odontostomatol. 2009 Dec 1;27(2):9-19.

Abstract

Teeth are amongst the most resilient elements of the human skeleton and are thus often utilized in routine forensic investigation involving the identification of unknown remains. Teeth exposed to thermal stress have the potential to not only aid in identification, but also in understanding the circumstances surrounding the fire. Children are twice as likely to become victims of house fire because of an inability to safely evacuate from areas of danger. The literature demonstrates, however, that research on the effects of incineration on teeth is mostly restricted to the permanent dentition. The apparent lack of knowledge on the effects of incineration on deciduous teeth thus necessitates further research in this area. To this end, this study aimed to relate colour changes that occur post heating with fragility to aid in proper handling of samples in a forensic scenario and to determine the possibility of identifying incineration temperature based on tooth condition. Teeth are amongst the most resilient elements of the human skeleton and are thus often utilized in routine forensic investigation involving the identification of unknown remains. Teeth exposed to thermal stress have the potential to not only aid in identification, but also in understanding the circumstances surrounding the fire. Children are twice as likely to become victims of house fire because of an inability to safely evacuate from areas of danger. The literature demonstrates, however, that research on the effects of incineration on teeth is mostly restricted to the permanent dentition. The apparent lack of knowledge on the effects of incineration on deciduous teeth thus necessitates further research in this area. To this end, this study aimed to relate colour changes that occur post heating with fragility to aid in proper handling of samples in a forensic scenario and to determine the possibility of identifying incineration temperature based on tooth condition. A total of 90 deciduous teeth, extracted as a part of routine clinical treatment, were exposed to temperatures ranging from 100°C to 1100°C for 30 minutes using a laboratory Gallenkamp oven. Unheated deciduous teeth were used as controls for the project. Post-incineration the teeth were analyzed under a stereomicroscope and SEM to assess the morphological changes. A colorimetric assessment was also undertaken to evaluate colour changes due to thermal stress. It was possible to identify incineration temperature based on tooth condition when the colour changes, stereomicroscopic findings and SEM images were utilized collectively. It was concluded that thermally induced changes in primary teeth occur at lower temperatures in comparison to the permanent teeth. It was also established that post-incineration deciduous teeth are fragile and show a tendency to fragment after minimal exposure to thermal stress.

摘要

牙齿是人体骨骼中最具韧性的部分之一,因此在涉及未知遗骸身份鉴定的常规法医调查中经常被使用。暴露于热应力下的牙齿不仅有助于身份鉴定,还能帮助了解火灾发生的情况。由于无法安全撤离危险区域,儿童成为房屋火灾受害者的可能性是成年人的两倍。然而,文献表明,关于焚烧对牙齿影响的研究大多局限于恒牙。显然,目前对焚烧对乳牙影响的了解不足,因此有必要在这一领域开展进一步研究。为此,本研究旨在将加热后发生的颜色变化与脆性联系起来,以帮助在法医场景中正确处理样本,并确定根据牙齿状况识别焚烧温度的可能性。牙齿是人体骨骼中最具韧性的部分之一,因此在涉及未知遗骸身份鉴定的常规法医调查中经常被使用。暴露于热应力下的牙齿不仅有助于身份鉴定,还能帮助了解火灾发生的情况。由于无法安全撤离危险区域,儿童成为房屋火灾受害者的可能性是成年人的两倍。然而,文献表明,关于焚烧对牙齿影响的研究大多局限于恒牙。显然,目前对焚烧对乳牙影响的了解不足,因此有必要在这一领域开展进一步研究。为此,本研究旨在将加热后发生的颜色变化与脆性联系起来,以帮助在法医场景中正确处理样本,并确定根据牙齿状况识别焚烧温度的可能性。总共90颗作为常规临床治疗一部分拔除的乳牙,使用实验室加伦坎普烤箱在100°C至1100°C的温度下暴露30分钟。未加热的乳牙用作该项目的对照。焚烧后牙齿在立体显微镜和扫描电子显微镜下进行分析,以评估形态变化。还进行了比色评估,以评估热应力引起的颜色变化。当综合利用颜色变化、立体显微镜观察结果和扫描电子显微镜图像时,可以根据牙齿状况识别焚烧温度。得出的结论是,与恒牙相比,乳牙在较低温度下就会发生热诱导变化。还发现,焚烧后的乳牙很脆弱,在受到最小热应力后就有破碎的倾向。

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