无需标记的荧光比率型探针用于检测多核苷酸激酶活性。
Label free fluorescence turn-on detection of polynucleotide kinase activity with a perylene probe.
机构信息
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.
出版信息
Chem Commun (Camb). 2012 Aug 14;48(63):7862-4. doi: 10.1039/c2cc32644b. Epub 2012 Jul 2.
PMID:22786467
Abstract
A single stranded oligonucleotide could induce aggregation of a perylene probe, the probe's monomer fluorescence was efficiently quenched. However, when the oligonucleotide was 5'-phosphorylated by polynucleotide kinase, it could be very efficiently degraded by lambda exonuclease, probe monomers were released, and a turn on fluorescence signal was detected.
摘要
单链寡核苷酸可以诱导芘探针的聚集，探针的单体荧光被有效地猝灭。然而，当寡核苷酸被多核苷酸激酶 5' - 磷酸化时，它可以被 λ 外切核酸酶非常有效地降解，探针单体被释放，并检测到荧光信号的开启。
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