Ma Changbei, Jin Shunxin, Liu Haisheng, Xia Kun, Tang Jianhua, Wang Kemin, Wang Jun
State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha 410013, China; State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410081, China.
State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha 410013, China.
Mol Cell Probes. 2015 Dec;29(6):500-502. doi: 10.1016/j.mcp.2015.11.002. Epub 2015 Nov 11.
We have developed a new methodology for label-free fluorescence turn-on detection of T4 polynucleotide kinase/phosphatase activity (T4 PNKP) using a Thioflavin T probe. This method is very sensitive with a 0.01 unit/mL limit of detection, which is better than those with labeled fluorophores. Furthermore, T4 PNKP inhibition by the inhibitor heparin is shown, demonstrating the potential to screen suitable inhibitor drugs for T4 PNKP.
我们开发了一种使用硫黄素T探针进行无标记荧光开启检测T4多核苷酸激酶/磷酸酶活性（T4 PNKP）的新方法。该方法非常灵敏，检测限为0.01单位/毫升，优于那些使用标记荧光团的方法。此外，还展示了抑制剂肝素对T4 PNKP的抑制作用，证明了筛选适合T4 PNKP的抑制剂药物的潜力。
Zotero 插件