Holland Mitchell M
Pennsylvania State University, University Park, Pennsylvania, USA.
Curr Protoc Hum Genet. 2012 Jul;Chapter 14:Unit14.7. doi: 10.1002/0471142905.hg1407s74.
This unit highlights methods used to perform PCR amplification and sequence analysis of mitochondrial DNA (mtDNA) on pristine and highly degraded biological material. The focus is on applications to forensic casework, and a number of case examples are provided. Any laboratory working with DNA from old or "ancient" samples will benefit from this information.
本单元重点介绍了对原始和高度降解的生物材料进行线粒体DNA(mtDNA)的PCR扩增及序列分析所使用的方法。重点在于法医案件工作中的应用,并提供了一些案例示例。任何处理来自陈旧或“古代”样本的DNA的实验室都将从这些信息中受益。