Piao A Z, Nojiri C, Park K D, Jacobs H, Feijen J, Kim S W
Department of Pharmaceutics, University of Utah, Salt Lake 84112.
J Biomater Sci Polym Ed. 1990;1(4):299-313. doi: 10.1163/156856289x00163.
Heparin and poly(ethylene oxide) were coupled to a central anchoring block of poly(dimethylsiloxane) in order to investigate its blood compatible properties. Diamino telechelic poly(dimethylsiloxane) (PDMS-(NH2)2, Mw = 20,000) was first modified to isocyanate functionalities using toluene 2,4-diisocyanate. This modified PDMS was then coupled to diamino-telechelic poly(ethylene oxide) (PEO-(NH2)2, Mw = 2000, 4000, 6000) to create BAB type copolymers having terminal free amino groups. These amino groups were covalently coupled to heparin containing terminal aldehyde groups using sodium cyanoborohydride to yield a bioactive, CBABC type block copolymer. The physical characterization of these copolymers was performed with IR, NMR, sulphur elemental analysis, Wilhelmy plate contact angle, and differential scanning calorimetry (DSC). CBABC block copolymer surfaces demonstrated heparin bioactivity in in vitro evaluation, and improved nonthrombogenic properties during ex vivo A-A shunt experiments.
为了研究其血液相容性，将肝素和聚环氧乙烷连接到聚二甲基硅氧烷的中央锚定嵌段上。首先使用甲苯2,4-二异氰酸酯将二氨基遥爪聚二甲基硅氧烷（PDMS-(NH2)2，Mw = 20,000）改性为异氰酸酯官能团。然后将这种改性的PDMS与二氨基遥爪聚环氧乙烷（PEO-(NH2)2，Mw = 2000、4000、6000）偶联，以制备具有末端游离氨基的BAB型共聚物。使用氰基硼氢化钠将这些氨基与含有末端醛基的肝素共价偶联，得到具有生物活性的CBABC型嵌段共聚物。通过红外光谱（IR）、核磁共振（NMR）、硫元素分析、威尔海姆板接触角和差示扫描量热法（DSC）对这些共聚物进行了物理表征。CBABC嵌段共聚物表面在体外评估中表现出肝素生物活性，并且在体外A-A分流实验中改善了抗血栓形成性能。
