Netherlands Institute for Innovative Ocular Surgery, Rotterdam, The Netherlands.
Cornea. 2013 Mar;32(3):285-9. doi: 10.1097/ICO.0b013e318253b1a6.
To study the validity of endothelial polymegethism, pleomorphism, and "poor swelling" as tissue discard parameters in the immediate postmortem evaluation of human donor corneal endothelium.
We retrospectively evaluated the quality of the endothelium at first and second evaluations for all processed corneas exhibiting moderate polymegethism, pleomorphism, or "poor swelling" in our eye bank over a 5-year period.
Out of 2008 eyes qualifying for our study, 422 corneas (21%) showed polymegethism, pleomorphism, or poor swelling at the first tissue evaluation immediately after excision of the corneoscleral button. In 363 (86%) of these corneas, a normal endothelial mosaic was observed at the second tissue evaluation after 7 to 21 days of organ culture, whereas only 59 (14%) still showed persistent polymegethism, pleomorphism, or "poor swelling" at that time point.
A recovery of normal endothelial cell mosaic and "normal swelling" at the second evaluation suggests that cellular contour parameters do not relate to tissue viability, but rather to a cellular stress reaction. If so, the validity of endothelial cellular contour morphology as an early parameter in assessing the suitability of a donor cornea for transplantation may be reconsidered.
研究内皮细胞多形性、异形性和“肿胀不良”作为人供体角膜内皮细胞即时死后评估中组织丢弃参数的有效性。
我们回顾性评估了在我们的眼库中，在 5 年期间，所有表现出中度多形性、异形性或“肿胀不良”的经处理角膜在第一次和第二次评估中的内皮质量。
在符合我们研究条件的 2008 只眼中，422 只角膜（21%）在切除角巩膜纽扣后立即进行的第一次组织评估中表现出多形性、异形性或肿胀不良。在这些角膜中，有 363 只（86%）在器官培养 7 至 21 天后的第二次组织评估中观察到正常的内皮镶嵌，而只有 59 只（14%）仍然表现出持续的多形性、异形性或“肿胀不良”。
在第二次评估中观察到正常内皮细胞镶嵌和“正常肿胀”的恢复表明，细胞轮廓参数与组织活力无关，而与细胞应激反应有关。如果是这样，那么将内皮细胞轮廓形态作为评估供体角膜移植适用性的早期参数的有效性可能需要重新考虑。
