Department of Biotechnology, Indian Institute of Technology, Roorkee, 247 667, Uttaranchal, India.
Plant Cell Rep. 2012 Oct;31(10):1779-87. doi: 10.1007/s00299-012-1291-3. Epub 2012 Jul 12.
A T-DNA insertional mutant OsPE of rice gives twin and triplet seedlings in up to 20 % of the seeds. Detailed cytological and histological analysis of OsPE indicated normal male and female gametogenesis in the OsPE mutant. Confocal laser scanning microscopic (CLSM) analysis of the developing seeds of OsPE showed multiple embryo development in up to 60 % of the ovules. The multiple embryos, mostly twins and triplets, and rarely quadruplets, developed through sequential cleavage from a single zygotic embryo in each ovule. The reduced number of multiple seedlings compared with multiple embryos observed in CLSM study may be attributed to their inability to develop further due to competition in a single embryo sac. Key message Multiple seedlings in the OsPE mutant are due to sequential proliferation and cleavage of the zygotic embryos. The nucellar tissue was not involved in multiple embryo development.
在高达 20%的种子中，水稻 T-DNA 插入突变体 OsPE 产生双生和三生子苗。对 OsPE 突变体的详细细胞学和组织学分析表明，其雄性和雌性配子体发生正常。对 OsPE 发育种子的共聚焦激光扫描显微镜 (CLSM) 分析显示，多达 60%的胚珠中存在多个胚胎发育。这些多个胚胎主要是双胞胎和三胞胎，很少是四胞胎，它们通过单个胚珠中单个合子胚胎的连续分裂发育而来。在 CLSM 研究中观察到的多个幼苗数量比多个胚胎少，这可能是由于它们在单个胚囊中的竞争而无法进一步发育所致。关键信息 OsPE 突变体中的多个幼苗是由于合子胚胎的连续增殖和分裂所致。珠心组织未参与多个胚胎的发育。
