van Roon M A, Zonneveld D, de Boer P A, Moorman A F, Charles R, Lamers W H
Department of Anatomy and Embryology, University of Amsterdam, The Netherlands.
Biol Neonate. 1990;58(3):152-9. doi: 10.1159/000243255.
The aim of this study was to see whether the rat embryo can serve as a model system for hepatocyte-specific gene expression in the human embryo. Carbamoylphosphate synthetase was used as a hepatocyte-specific marker molecule. Despite the earlier developmental appearance of this enzyme in human than in murine liver, the hormonal regulation of gene expression in cultures of embryonic hepatocytes was found to be the same. Therefore, a relatively early developmental appearance of regulatory hormones rather than differences in regulatory mechanisms of gene expression appears to be responsible for the early accumulation of the enzyme in human liver, when compared to murine liver.
本研究的目的是探究大鼠胚胎是否可作为人类胚胎中肝细胞特异性基因表达的模型系统。氨甲酰磷酸合成酶被用作肝细胞特异性标记分子。尽管该酶在人类肝脏中的发育出现时间早于小鼠肝脏，但胚胎肝细胞培养物中基因表达的激素调节被发现是相同的。因此，与小鼠肝脏相比，调节激素相对较早的发育出现而非基因表达调节机制的差异似乎是该酶在人类肝脏中早期积累的原因。
