完整组织-金属界面的超微结构研究方法。
Method for ultrastructural studies of the intact tissue-metal interface.
作者信息
Bjursten L M, Emanuelsson L, Ericson L E, Thomsen P, Lausmaa J, Mattsson L, Rolander U, Kasemo B
机构信息
Department of Anatomy, University of Gothenburg, Sweden.
出版信息
Biomaterials. 1990 Oct;11(8):596-601. doi: 10.1016/0142-9612(90)90085-5.
Samples were prepared for ultrastructural studies of the intact interface between metallic implants and tissue by transmission electron microscopy. The method is based on plastic embedding of implant and tissue and subsequent removal of the bulk metal by electrochemical dissolution (electropolishing), to facilitate preparation of ultrathin sections for transmission electron microscopy. Surface sensitive spectroscopy (Auger electron microscopy and X-ray photoemission spectroscopy) and transmission electron microscopy EDX results show that the method produces samples with an intact interface, containing the implant surface oxide and the adjacent tissue. Examples of application of the method on titanium, zirconium and aluminium implants in soft tissue are given.
通过透射电子显微镜制备用于金属植入物与组织完整界面超微结构研究的样本。该方法基于对植入物和组织进行塑料包埋，随后通过电化学溶解（电解抛光）去除大量金属，以利于制备用于透射电子显微镜的超薄切片。表面敏感光谱（俄歇电子显微镜和X射线光电子能谱）以及透射电子显微镜能谱分析结果表明，该方法制备出的样本具有完整界面，包含植入物表面氧化物和相邻组织。文中给出了该方法在软组织中钛、锆和铝植入物上的应用实例。
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