Yamasaki Masao, Iwase Masahiro, Kawano Kazuo, Sakakibara Yoichi, Suiko Masahito, Nishiyama Kazuo
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
Biosci Biotechnol Biochem. 2012;76(6):1177-81. doi: 10.1271/bbb.120061. Epub 2012 Jun 7.
We determined the effects of apocynin, a representative inhibitor of NADPH oxidase, on the proliferative and adhesive properties of 3Y1 rat fibroblasts and the 3Y1 v-H-ras-transformed derivative, HR-3Y1-2. Apocynin inhibited the proliferation of HR-3Y1-2 but not 3Y1 cells at 10 µM and 100 µM. Apocynin also decreased the intracellular reactive oxygen species (ROS) level in HR-3Y1-2 but not 3Y1 cells. We also evaluated the effects of apocynin on cell adhesion to fibronectin and found decreased adhesion of HR-3Y1-2 cells to fibronectin-coated plates. Our results indicate that apocynin selectively down-regulated β1-integrin cell surface expression on the HR-3Y1-2 cells. It also inhibited the migration and invasion of these cells. These data suggest that reducing the production of NADPH oxidase-mediated ROS could be an effective means for ameliorating the abnormal growth, adhesion and motility of v-H-ras-transformed cells.
我们确定了NADPH氧化酶的代表性抑制剂白杨素对3Y1大鼠成纤维细胞及其v-H-ras转化衍生物HR-3Y1-2的增殖和黏附特性的影响。白杨素在10 μM和100 μM时抑制HR-3Y1-2细胞的增殖，但不抑制3Y1细胞的增殖。白杨素还降低了HR-3Y1-2细胞内活性氧（ROS）水平，但不降低3Y1细胞内的ROS水平。我们还评估了白杨素对细胞与纤连蛋白黏附的影响，发现HR-3Y1-2细胞与纤连蛋白包被平板的黏附减少。我们的结果表明，白杨素选择性地下调了HR-3Y1-2细胞表面β1整合素的表达。它还抑制了这些细胞的迁移和侵袭。这些数据表明，减少NADPH氧化酶介导的ROS产生可能是改善v-H-ras转化细胞异常生长、黏附和运动的有效手段。
