Chen Yun
Wallenberg Laboratory, Department of Molecular and Clinical Medicine/Clinical Physiology, The Sahlgrenska Academy and University Hospital, University of Gothenburg, Gothenburg, Sweden.
Methods Mol Biol. 2012;906:193-8. doi: 10.1007/978-1-61779-953-2_14.
Quantum dots (QDs) are semiconductor nanocrystals with unique photo-physical properties. QDs are brightly fluorescent and photostable, and therefore represent a novel class of fluorescence probes for bio-imaging and cell monitoring. There are different techniques for labeling cells with QDs. Moreover, there is evidence that optical properties of the QD can be changed as a result of interaction with live cells. This manuscript describes a simple method for labeling of rat endothelial progenitor cells using ODs and for characterization of their optical properties.
量子点（QDs）是具有独特光物理性质的半导体纳米晶体。量子点具有明亮的荧光且光稳定性好，因此代表了一类用于生物成像和细胞监测的新型荧光探针。有多种用量子点标记细胞的技术。此外，有证据表明量子点的光学性质会因与活细胞相互作用而改变。本手稿描述了一种使用量子点标记大鼠内皮祖细胞并表征其光学性质的简单方法。
