Pantazis Panayotis, Dimas Konstantinos, Wyche James H, Anant Shrikant, Houchen Courtney W, Panyam Jayanth, Ramanujam Rama P
ADNA, Inc, Oklahoma City, OK, USA.
Methods Mol Biol. 2012;906:311-9. doi: 10.1007/978-1-61779-953-2_25.
Nanoparticles (NPs) formulated using poly (D,L-lactide-co-glycolide) (PLGA), a biodegradable, biocompatible, and clinically approved polymer, have been widely used for targeted drug delivery. Here we provide methods for preparing PLGA NPs that encapsulate small interfering RNA (siRNA). The siRNA NPs are formulated using a double-emulsion solvent evaporation technique with the addition of a small amount of the cationic polymer, polyethyleneimine, which significantly increases siRNA encapsulation.
使用聚（D，L-丙交酯-共-乙交酯）（PLGA）制备的纳米颗粒（NPs），PLGA是一种可生物降解、生物相容且已获临床批准的聚合物，已被广泛用于靶向给药。在此，我们提供了制备包裹小干扰RNA（siRNA）的PLGA NPs的方法。siRNA NPs采用双乳液溶剂蒸发技术，并添加少量阳离子聚合物聚乙烯亚胺来制备，这显著提高了siRNA的包封率。
