Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
IET Nanobiotechnol. 2013 Jun;7(2):33-41. doi: 10.1049/iet-nbt.2012.0012.
Linamarin-loaded poly (lactide-co-glycolide) (PLGA) nanoparticles (NPs) were prepared by the double emulsion solvent evaporation technique. The formulated PLGA (50:50) and PLGA (85:15) NPs were spherically shaped, having an average particle size < 190 nm, drug entrapment efficiency (50-52%) and zeta potentials ranging from -25 to -30 mV. Interestingly, all formulated PLGA NPs exhibited a controlled biphasic release profile. Polymer degradation was investigated in the current research to determine the major degradation products and then the polymer biocompatibility as well as safety. The PLGA NPs degradation behaviour was investigated by measuring water uptake, mass loss, change of pH of the degradation medium, morphological changes, and lactic and glycolic acid concentrations. Gravimetrical methods, pH meter, scanning electron microscope and high-performance liquid chromatography were employed, respectively. PLGA (50:50) NPs were found to degrade faster than PLGA (85:15) NPs. With regard to water uptake, mass loss and pH change, the degradation behaviour of PLGA (50:50) NPs was significantly (rho < 0.05) different from that of PLGA (85:15) NPs. A complete degradation of PLGA (50:50) NPs was achieved after 102 days, whereas, only about 60% of PLGA (85:15) NPs were degraded within the same period. Complete degradation and release of the degradation products naturally by the body ensures safety of the delivery carrier.
用双乳液溶剂蒸发技术制备了负载黎豆氨酸的聚（乳酸-共-乙醇酸）（PLGA）纳米粒子（NPs）。所制备的 PLGA（50:50）和 PLGA（85:15）NPs 呈球形，平均粒径<190nm，药物包封效率（50-52%）和zeta 电位在-25 到-30mV 之间。有趣的是，所有的 PLGA NPs 均表现出一种控制的两相释放模式。在当前的研究中，对聚合物降解进行了研究，以确定主要的降解产物，然后评估聚合物的生物相容性和安全性。通过测量水摄取、质量损失、降解介质 pH 值的变化、形态变化以及乳酸和乙醇酸的浓度，研究了 PLGA NPs 的降解行为。分别采用重量法、pH 计、扫描电子显微镜和高效液相色谱法。PLGA（50:50）NPs 的降解速度明显快于 PLGA（85:15）NPs。就水摄取、质量损失和 pH 值变化而言，PLGA（50:50）NPs 的降解行为与 PLGA（85:15）NPs 有显著差异（rho < 0.05）。PLGA（50:50）NPs 在 102 天后完全降解，而在相同时间内，只有大约 60%的 PLGA（85:15）NPs 降解。PLGA（50:50）NPs 的完全降解和降解产物的自然释放可确保载体的安全性。
