Pfeiffer G, Geyer H, Geyer R, Kalsner I, Wendorf P
Biochemisches Institut am Klinikum, Universität Giessen, FRG.
Biomed Chromatogr. 1990 Sep;4(5):193-9. doi: 10.1002/bmc.1130040505.
A series of high-mannose and complex type glycoprotein-N-glycans was subjected to high-pH anion-exchange chromatography. The results revealed that this method represents a useful tool for analytical characterization of single oligosaccharides as well as preparative separation of complex mixtures of carbohydrate side-chains. On the other hand, it became evident that in several cases a combination of different chromatographic techniques is required for efficient separation of individual oligosaccharide species.
一系列高甘露糖型和复合型糖蛋白 - N - 聚糖被用于高pH值阴离子交换色谱分析。结果表明，该方法是用于单糖分析表征以及碳水化合物侧链复杂混合物制备分离的有用工具。另一方面，很明显在几种情况下需要结合不同的色谱技术才能有效分离各个寡糖种类。
