L-甘油-D-甘露庚吡喃糖和-D-甘油-D-甘露庚糖醇的部分甲基化和乙酰化衍生物的气相色谱-质谱分析

G.l.c.-m.s. of partially methylated and acetylated derivatives of L-glycero-D-manno- and D-glycero-D-manno-heptopyranoses and -heptitols.

作者信息

Holst O, Brade H, Dziewiszek K, Zamojski A

机构信息

Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, F.R.G.

出版信息

Carbohydr Res. 1990 Sep 5;204:1-9. doi: 10.1016/0008-6215(90)84016-n.

DOI:10.1016/0008-6215(90)84016-n

PMID:2279240

Abstract

Methylated or acetylated heptopyranose derivatives were prepared variously from L-glycero-D-manno- and D-glycero-D-manno-heptopyranose, O-L- glycero-alpha-D-manno-heptopyranosyl-(1----3)-L-glycero-D-manno- heptopyranose, O-L-glycero-alpha-D-manno-heptopyranosyl-(1----7)-L-glycero- D-manno-heptopyranose, and O-L-glycero-alpha-D-manno- heptopyranosyl-(1----7)-O-L-glycero-alpha-D-manno- heptopyranosyl-(1----3)-L-glycero-D-manno- heptopyranose, which are structural elements of the heptose region of enterobacterial lipopolysaccharides. Each derivative was investigated by g.l.c. and g.l.c.-m.s., and the retention times and fragmentation patterns were used to identify partial structures of the heptose region of the core oligosaccharide of bacterial LPS.

摘要

甲基化或乙酰化的庚吡喃糖衍生物可由L-甘油-D-甘露庚吡喃糖、D-甘油-D-甘露庚吡喃糖、O-L-甘油-α-D-甘露庚吡喃糖基-(1→3)-L-甘油-D-甘露庚吡喃糖、O-L-甘油-α-D-甘露庚吡喃糖基-(1→7)-L-甘油-D-甘露庚吡喃糖以及O-L-甘油-α-D-甘露庚吡喃糖基-(1→7)-O-L-甘油-α-D-甘露庚吡喃糖基-(1→3)-L-甘油-D-甘露庚吡喃糖以多种方式制备，这些都是肠道细菌脂多糖庚糖区域的结构元件。通过气相色谱法（g.l.c.）和气相色谱-质谱联用（g.l.c.-m.s.）对每种衍生物进行了研究，并利用保留时间和碎片模式来鉴定细菌脂多糖核心寡糖庚糖区域的部分结构。