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从大肠杆菌K-12和明尼苏达沙门氏菌脂多糖的内核区域分离并鉴定3-脱氧-D-甘露-2-辛酮糖酸7-(2-氨基乙基磷酸酯)

Isolation and characterisation of 3-deoxy-D-manno-2-octulopyranosonate 7-(2-aminoethyl phosphate) from the inner core region of Escherichia coli K-12 and Salmonella minnesota lipopolysaccharides.

作者信息

Holst O, Röhrscheidt-Andrzejewski E, Brade H

机构信息

Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, F.R.G.

出版信息

Carbohydr Res. 1990 Sep 5;204:93-102. doi: 10.1016/0008-6215(90)84024-o.

DOI:10.1016/0008-6215(90)84024-o
PMID:2279250
Abstract

The title compound (PE-Kdo) was isolated after hydrolysis of the lipopolysaccharides of Escherichia coli K-12 strain W3100 and Salmonella minnesota strains R4 and R7, and the location of the 2-aminoethyl phosphate group at position 7 was established by 13C-n.m.r. spectroscopy. Derivatives of PE-Kdo were acetylated, silylated, and methylated in order to evaluate their usefulness for analysis by g.l.c.-m.s.

摘要

标题化合物（PE-Kdo）是在大肠杆菌K-12菌株W3100以及明尼苏达沙门氏菌菌株R4和R7的脂多糖水解后分离得到的，通过碳-13核磁共振光谱确定了7位上2-氨基乙基磷酸基团的位置。对PE-Kdo的衍生物进行了乙酰化、硅烷化和甲基化处理，以评估它们在气相色谱-质谱联用分析中的适用性。

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Studies on the reductive amination of 3-deoxy-D-manno-octulosonic acid (Kdo).3-脱氧-D-甘露糖辛酸（Kdo）的还原胺化研究。
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Genes for TDP-rhamnose synthesis affect the pattern of lipopolysaccharide heterogeneity in Escherichia coli K-12.
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