Wang Qiuling, Wang Wenquan, Wei Shengli, Yu Fulai, Peng Fang, Fang Yuqiang
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Zhongguo Zhong Yao Za Zhi. 2012 Apr;37(7):920-4.
To study on the effect of different processing methods on the contents of seven major constituents in wild and cultivated Paeonia lactiflora, gallic acid, catechin, albiflorin, paeoniflorin, pentagalloylglucose, benzoic acid and paeonol, in order to provide reference basis for different efficacy and formation mechanism of Paeonia Radix Rubra and Paeonia Radix Alba.
Wild and cultivated P. lactiflora were dealt with by four processing methods, direct drying, drying after boiling, drying after decorticating and boiling, and drying after boiling and decorticating. HPLC was use to simultaneously determine the contents of seven chemical constituents.
Wild P. lactiflora showed notable higher content of paeoniflorin and catechin than cultivated P. lactiflora, whereas cultivated P. lactiflora showed higher content of albiflorin than wild P. lactiflora. Both of them were less affected by process methods in above three constituents. Drying after boiling, drying after decorticating and boiling, and drying after boiling and decorticating methods reduced the content of benzoic acid and paeonol to trace in both wild and cultivated P. lactiflora. Clustering analysis results showed that all processing methods assembled wild and cultivated P. lactiflora in 2 groups.
The content differences of Paeonia Radix Rubra and Paeonia Radix Alba are mainly caused by their own differences and less affected by processing methods.
研究不同炮制方法对野生与栽培芍药中没食子酸、儿茶素、白花芍药苷、芍药苷、五倍子酰葡萄糖、苯甲酸和丹皮酚七种主要成分含量的影响，为赤芍和白芍不同功效及形成机制提供参考依据。
采用直接干燥、煮后干燥、去皮煮后干燥、煮后去皮干燥四种炮制方法对野生与栽培芍药进行处理，用高效液相色谱法同时测定七种化学成分的含量。
野生芍药中芍药苷和儿茶素的含量显著高于栽培芍药，而栽培芍药中白花芍药苷的含量高于野生芍药。上述三种成分受炮制方法的影响较小。煮后干燥、去皮煮后干燥、煮后去皮干燥法均使野生与栽培芍药中苯甲酸和丹皮酚的含量降至微量。聚类分析结果表明，所有炮制方法将野生与栽培芍药聚为两组。
赤芍和白芍的含量差异主要由其自身差异引起，受炮制方法的影响较小。
