[高效液相色谱法同时测定血竭及其提取物中的五种活性成分]

[Simultaneous determination of five active components in resina draconis and its extract by HPLC].

作者信息

Li Yun, Xiao Wei, Qin Jianping, Guo Ying, Zhu Qianqian

机构信息

Nanjing University of Chinese Medicine, Jiangsu 210000, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2012 Apr;37(7):929-33.

PMID:22792791

Abstract

OBJECTIVE

To establish an HPLC method for simultaneous determination of contents of loureirin A, loureirin B, 7,4'-dihydroxy flavone, pterostilbene and resveratrol in resina draconis and its extracts.

METHOD

Kromasil 100-5C18 column (4.6 mm x 250 mm, 5 microm) was used with the mobile phase of acetonitrile-1% glacial acetic acid at a flow rate of 1.0 mL x min(-1) and the column temperature at 40 degrees C. The detective wave length of loureirin A and B was detected at 278 nm, and 7,4'-dihydroxy flavone, pterostilbene and resveratrol was at 319 nm.

RESULT

All the five active components reached the resolved peaks within 40 min, indicating a good linearity (r > or = 0.999 7). The average recoveries of loureirin A, loureirin B, 7,4'-dihydroxy flavone, pterostilbene and resveratrol in resina draconis were 102.9%, 96.81%, 97.29%, 100.7% and 103.7%, with RSDs of 0.23%, 1.5%, 0.42%, 0.58% and 0.34%, respectively. The average recoveries of loureirin A, loureirin B, 7,4'-dihydroxy flavone, pterostilbene and resveratrol in extract of resina draconis were 102. 2% , 96. 93%, 97. 90% , 102.0% and 103.3%, with RSDs of 1.7%, 0.91%, 1.4%, 1.5% and 1.2%, respectively.

CONCLUSION

The method is so easy, accurate, highly repeatable and stable that it provides good reference for the quality control of resina draconis and its extracts.

摘要

目的

建立高效液相色谱法同时测定血竭及其提取物中龙血素A、龙血素B、7,4'-二羟基黄酮、紫檀芪和白藜芦醇的含量。

方法

采用Kromasil 100-5C18色谱柱（4.6 mm×250 mm，5μm），以乙腈-1%冰醋酸为流动相，流速为1.0 mL·min⁻¹，柱温40℃。龙血素A和B的检测波长为278 nm，7,4'-二羟基黄酮、紫檀芪和白藜芦醇的检测波长为319 nm。

结果

5种活性成分在40 min内均达到分离峰，线性关系良好（r≥0.999 7）。血竭中龙血素A、龙血素B、7,4'-二羟基黄酮、紫檀芪和白藜芦醇的平均回收率分别为102.9%、96.81%、97.29%、100.7%和103.7%，相对标准偏差分别为0.23%、1.5%、0.42%、0.58%和0.34%。血竭提取物中龙血素A、龙血素B、7,4'-二羟基黄酮、紫檀芪和白藜芦醇的平均回收率分别为102.2%、96.93%、97.90%、102.0%和103.3%，相对标准偏差分别为1.7%、0.91%、1.4%、1.5%和1.2%。