Fluhr R
Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel.
Dev Genet. 1990;11(3):197-204. doi: 10.1002/dvg.1020110305.
Transgenic plants have been particularly useful in studying nuclear genes encoding for photosynthetic functions. The expression of these genes and their chimeric constructs in transgenic plants faithfully mimics their natural counterparts. The use of sensitive chimeric reporter genes has enabled localizing the activity of genes encoding photosynthetic proteins to individual cells. Cab and rbcS transgenes have been shown to retain sensitivity to light quality, which is modulated by phytochrome. Conditional light activation under the influence of a circadian rhythm has been shown for Cab transgenes. Transgenic plants containing truncated promoters have helped delineate cis-regulatory positive and negative elements involved in light-mediated transcriptional induction and tissue specificity.
转基因植物在研究编码光合功能的核基因方面特别有用。这些基因及其嵌合构建体在转基因植物中的表达忠实地模拟了它们的天然对应物。使用敏感的嵌合报告基因能够将编码光合蛋白的基因活性定位到单个细胞。已证明Cab和rbcS转基因对光质保持敏感性，而光质受光敏色素调节。已证明Cab转基因在昼夜节律的影响下有条件地光激活。含有截短启动子的转基因植物有助于描绘参与光介导的转录诱导和组织特异性的顺式调节正、负元件。
