Institut de Biochimie Moléculaire et Cellulaire (IBBMC), Université Paris-Sud, CNRS, UMR 8619, Orsay, F-91405 Orsay, France.
Trends Biotechnol. 2012 Oct;30(10):512-20. doi: 10.1016/j.tibtech.2012.06.001. Epub 2012 Jul 13.
How do we create new artificial proteins? In this review, we present a range of experimental approaches based on combinatorial and directed evolution methods used to explore sequence space and recreate structured or active proteins. These approaches can help to understand constraints of natural evolution and can lead to new useful proteins. Strategies such as binary patterning or modular assembly can efficiently speed structural and functional innovation. Many natural protein architectures are symmetric or repeated and presumably have emerged by coalescence of simpler fragments. This process can be experimentally reproduced; a range of artificial proteins obtained from idealized fragments has recently been described and some of these have already found direct applications.
我们如何创造新的人工蛋白质？在这篇综述中，我们提出了一系列基于组合和定向进化方法的实验方法，用于探索序列空间并重新创建结构或活性蛋白质。这些方法可以帮助我们理解自然进化的限制，并可能导致新的有用蛋白质。例如二进制图案化或模块化组装等策略可以有效地加速结构和功能的创新。许多天然蛋白质结构是对称的或重复的，并且推测是通过简单片段的合并而出现的。这个过程可以通过实验来重现；最近已经描述了一系列从理想化片段获得的人工蛋白质，其中一些已经找到了直接的应用。
