Oral Biology Department, Cairo University, Cairo, Egypt.
Arch Oral Biol. 2012 Oct;57(10):1342-54. doi: 10.1016/j.archoralbio.2012.06.007. Epub 2012 Jul 12.
Activation of receptor for advanced glycation end products (RAGE) has been implicated in many chronic diseases, including diabetic complications. In this study we examined the relationship between RAGE expression and the morphological changes seen in the gingiva of diabetic and periodontitis patients.
Gingival biopsies from 15 diabetic patients with periodontitis, 25 non diabetic patients with periodontitis and 10 healthy individuals were collected. Sections were stained with haematoxylin and eosin, and immunohistochemically to detect RAGE. Samples were examined in light and fluorescence microscopes and histomorphometric analysis was performed.
Increased number of inflammatory cells and changes in collagen and vasculature were observed in diabetic and non diabetic patients with periodontitis. RAGE was weakly expressed in healthy gingiva. The strongest reaction with anti-RAGE antibody was found in the gingiva of diabetic patients with periodontitis followed by the severe periodontitis patients. RAGE expression in inflammatory cells was stronger than in the epithelium. The number of inflammatory cells in the gingiva was higher in the diabetic periodontitis patients than in the non diabetic severe periodontitis patients.
RAGE is strongly expressed in the gingiva of diabetic patients with periodontitis and with severe periodontitis alone, the latter indicating RAGE activation even in the absence of hyperglycemia. However our findings are based on relatively small sample size. With a larger patient population, some of our other findings may have reached statistical significance.
糖基化终产物受体（RAGE）的激活与许多慢性疾病有关，包括糖尿病并发症。本研究检测了 RAGE 表达与糖尿病和牙周炎患者牙龈形态变化之间的关系。
收集了 15 例糖尿病伴牙周炎患者、25 例非糖尿病伴牙周炎患者和 10 例健康个体的牙龈活检组织。用苏木精和伊红染色和免疫组织化学法检测 RAGE。在光镜和荧光显微镜下观察标本，并进行组织形态计量学分析。
糖尿病和非糖尿病牙周炎患者的炎症细胞数量增加，胶原和血管发生变化。健康牙龈中 RAGE 表达较弱。糖尿病伴牙周炎患者的牙龈与严重牙周炎患者的 RAGE 反应最强。炎症细胞中的 RAGE 表达强于上皮细胞。糖尿病牙周炎患者的牙龈炎症细胞数量高于非糖尿病严重牙周炎患者。
RAGE 在糖尿病伴牙周炎患者的牙龈中强烈表达，在单纯严重牙周炎患者中也有表达，这表明即使没有高血糖，RAGE 也被激活。然而，我们的研究结果是基于相对较小的样本量。在更大的患者群体中，我们的一些其他发现可能达到了统计学意义。
