INRA, UMR 1347 Agroécologie, F-21065 Dijon cedex, France.
J Hazard Mater. 2012 Sep 15;231-232:88-97. doi: 10.1016/j.jhazmat.2012.06.041. Epub 2012 Jul 1.
Technosol construction through assemblage of treated soil and recycled wastes is an innovative option for the restoration of degraded lands and re-use of industrial wastes. Recent studies have evidenced that Technosols could support soil functions such as primary production but the knowledge about other ecosystemic services, such as nutrient cycling, is limited. We investigated how the total bacterial community and key functional microbial communities involved in nitrogen cycling were influenced by the depth and type of Technosol. We found that despite being artificially constructed, Technosols exhibited a gradual change in microbial activity and abundance along the soil profile. Both nitrification and denitrification rates decreased with increasing depth as previously observed in other soil systems. Potential denitrification and nitrification were correlated with Technosol physico-chemical properties but also with abundances of nirS denitrifiers and bacterial amoA gene, respectively. The correlation between nitrification rates and bacterial ammonia-oxidizers suggests that bacteria are driving nitrification in Technosols. A-RISA fingerprints showed a distinct community structure along different Technosol layers. Technosol properties affected denitrification strongly than nitrification underlining the importance of better understanding the microbial communities in Technosols to maximize their potential for nutrient cycling, an essential ecosystem function.
通过组合处理过的土壤和回收废物来建造技术溶胶是恢复退化土地和再利用工业废物的一种创新选择。最近的研究表明，技术溶胶可以支持土壤功能，如初级生产，但关于其他生态系统服务，如养分循环的知识有限。我们研究了总细菌群落和参与氮循环的关键功能微生物群落如何受到技术溶胶深度和类型的影响。我们发现，尽管是人工建造的，技术溶胶在土壤剖面中表现出微生物活性和丰度的逐渐变化。正如在其他土壤系统中观察到的那样，硝化和反硝化速率随着深度的增加而降低。潜在的反硝化和硝化与技术溶胶的理化性质有关，但也与nirS 反硝化菌和细菌 amoA 基因的丰度有关。硝化速率与细菌氨氧化菌之间的相关性表明，细菌是技术溶胶中硝化作用的驱动力。A-RISA 指纹图谱显示出不同技术溶胶层之间独特的群落结构。技术溶胶特性对反硝化的影响强于硝化，这强调了更好地理解技术溶胶中微生物群落的重要性，以最大限度地发挥其养分循环的潜力，这是一种重要的生态系统功能。
