利用巴贝斯虫属吉氏血巴尔通体重组血栓反应蛋白相关黏附蛋白建立一种快速免疫层析检测方法。
Development of a rapid immunochromatographic test using a recombinant thrombospondin-related adhesive protein of Babesia gibsoni.
机构信息
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
出版信息
Vet Parasitol. 2012 Dec 21;190(3-4):595-8. doi: 10.1016/j.vetpar.2012.06.024. Epub 2012 Jun 26.
We developed an immunochromatographic test (ICT) with the full-length of thrombospondin-related adhesive protein of Babesia gibsoni expressed by the modified expression method. The developed ICT showed high sensitivity, specificity, and kappa value with a reference test (100%, 93.78%, and 0.8976, respectively), indicating that the ICT could be a new practical diagnostic test for B. gibsoni infection.
我们采用改良表达方法，表达巴贝西原虫血小板反应蛋白相关黏附蛋白全长，研制免疫胶体金渗滤法检测试剂盒。该试剂盒与参考方法比较，具有较高的敏感性、特异性和符合率（分别为 100%、93.78%和 0.8976），表明该试剂盒有望成为一种新的实用的贝氏巴贝斯虫病诊断方法。
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