Department of Pathobiology and Diagnostic Investigation, Diagnostic Center for Population and Animal Health, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824-1314, USA.
Vet Microbiol. 2012 Dec 7;160(3-4):484-7. doi: 10.1016/j.vetmic.2012.06.028. Epub 2012 Jul 3.
Emerging evidence suggests that cats infected with feline herpesvirus-1 (FHV-1) may experience a brief viremic phase. The objective of this study was to determine whether natural routes of FHV-1 inoculation could result in viremic transmission of infectious virus to connective tissues (cortical bone, tendon). Three specific pathogen-free cats were experimentally inoculated with FHV-1 via a combined mucosal (oronasal, ocular) route. Cats were euthanized at the peak of clinical signs to aseptically harvest tissues (cortical bone, tendon, trachea/tongue) for co-culture with a susceptible cell line to promote spread of infectious virus. Viral infection of Crandall-Rees feline kidney cells was microscopically visualized by cytopathic effect (CPE). Additionally, co-culture DNA was extracted either at the point of CPE or 16 days of culture without evidence of CPE, to amplify FHV-1 glycoprotein B gene using real-time PCR. Infectious virus was detected in distant cortical bone (two cats, moderate to severe clinical signs) and tendon (one cat, severe clinical signs). Direct infection of mucosal (trachea, tongue) tissues also was confirmed in these two cats. In contrast, all co-cultured tissues from the third cat (mild clinical signs) were negative for FHV-1 by CPE and PCR. Results of this study demonstrated that early primary FHV-1 viremia may be distributed to distant connective tissues.
新出现的证据表明，感染猫疱疹病毒 1 型（FHV-1）的猫可能会经历短暂的病毒血症期。本研究的目的是确定 FHV-1 的自然接种途径是否会导致传染性病毒通过血管传播到结缔组织（皮质骨、肌腱）。将三只特定病原体无病猫通过联合粘膜（鼻内、眼部）途径进行 FHV-1 实验接种。在临床症状高峰时对猫进行安乐死，以无菌方式采集组织（皮质骨、肌腱、气管/舌）进行共培养，以促进传染性病毒的传播。通过细胞病变效应（CPE）观察到 Crandall-Rees 猫肾细胞中病毒感染。此外，在出现 CPE 或无 CPE 的 16 天培养时提取共培养 DNA，使用实时 PCR 扩增 FHV-1 糖蛋白 B 基因。在两只临床症状中度至重度的猫的远处皮质骨（two cats, moderate to severe clinical signs）和肌腱（one cat, severe clinical signs）中检测到传染性病毒。在这两只猫中，还直接感染了粘膜（气管、舌）组织。相比之下，在临床症状较轻的第三只猫的所有共培养组织中，CPE 和 PCR 均未检测到 FHV-1。本研究结果表明，早期原发性 FHV-1 病毒血症可能分布到远处的结缔组织。
