Fedorocko P, Brezáni P, Macková N
Institute of General Biology, Faculty of Science, P. J. Safárik University, Kosice.
Folia Biol (Praha). 1990;36(6):344-50.
Pretreatment with cell-free supernatant of thymus cell-conditioned medium (TCCM) protects mice in a dose-dependent manner from the lethal effect of ionizing radiation. A single dose of TCCM injected i.p. in a volume of 1.2 ml 8 h before lethal gamma irradiation with a dose of 9 Gy (LD95/30) protected 36% of C57BL/6 mice. Four doses of TCCM injected i.p. in a volume of 0.8 ml once a day (i.e., during four days), protected 50% of C57BL/6 mice. Application of five-fold concentrated supernatant of conditioned medium (cTCCM) in a volume of 1 ml i.p. 8 h before irradiation with a dose of 9 Gy protected 75% of mice. On the other hand, no therapeutic effect of different doses of the supernatant fluids from thymus cell cultures was found. Our observations suggest that media conditioned by thymus cells can mediate a radioprotective effect.
用胸腺细胞条件培养基（TCCM）的无细胞上清液进行预处理，可使小鼠以剂量依赖的方式免受电离辐射的致死效应。在9 Gy（LD95/30）致死性γ射线照射前8小时，以1.2 ml的体积腹腔注射单剂量TCCM，可保护36%的C57BL/6小鼠。每天一次（即连续四天）以0.8 ml的体积腹腔注射四剂TCCM，可保护50%的C57BL/6小鼠。在9 Gy剂量照射前8小时，以1 ml的体积腹腔注射五倍浓缩的条件培养基上清液（cTCCM），可保护75%的小鼠。另一方面，未发现不同剂量的胸腺细胞培养上清液具有治疗效果。我们的观察结果表明，胸腺细胞条件培养基可介导辐射防护作用。
