Chemistry Division, US Naval Research Laboratory, 4555 Overlook Avenue, SW, Washington, DC 20375, USA.
Biosens Bioelectron. 2013 Feb 15;40(1):102-9. doi: 10.1016/j.bios.2012.06.039. Epub 2012 Jun 29.
The genes involved in the proposed pathway for Shewanella extracellular electron transfer (EET) are highly conserved. While extensive studies involving EET from a fresh water Shewanella microbe (S. oneidensis MR-1) to soluble and insoluble electron acceptors have been published, only a few reports have examined EET from marine strains of Shewanella. Thus, Shewanella frigidimarina (an isolate from Antarctic Sea ice) was used within miniature microbial fuel cells (mini-MFC) to evaluate potential power output. During the course of this study several distinct differences were observed between S. oneidensis MR-1 and S. frigidimarina under comparable conditions. The maximum power density with S. frigidimarina was observed when the anolyte was half-strength marine broth (1/2 MB) (0.28 μW/cm(2)) compared to Luria-Bertani (LB) (0.07 μW/cm(2)) or a defined growth minimal medium (MM) (0.02 μW/cm(2)). The systematic modification of S. frigidimarina cultured in 1/2 MB and LB with divalent cations shows that a maximum current output can be generated independent of internal ionic ohmic losses and the presence of external mediators.
所提出的希瓦氏菌细胞外电子传递 (EET) 途径中涉及的基因高度保守。虽然已经发表了大量涉及来自淡水希瓦氏菌（S. oneidensis MR-1）到可溶性和不溶性电子受体的 EET 的研究，但只有少数报告研究了海洋希瓦氏菌菌株的 EET。因此，使用来自南极海冰的希瓦氏菌 frigidimarina（分离株）在微型微生物燃料电池（mini-MFC）中评估潜在的功率输出。在这项研究过程中，在可比条件下，观察到 S. oneidensis MR-1 和 S. frigidimarina 之间的几个明显差异。当阳极电解液为半强度海洋肉汤 (1/2 MB)（0.28 μW/cm(2)）时，观察到 S. frigidimarina 的最大功率密度与 Luria-Bertani (LB)（0.07 μW/cm(2)）或定义的生长最小培养基 (MM)（0.02 μW/cm(2)）相比。用二价阳离子系统地修饰在 1/2 MB 和 LB 中培养的 S. frigidimarina 表明，可以在不考虑内部离子欧姆损耗和外部介体存在的情况下产生最大电流输出。
