UMR Agroécologie 1347, AgroSup/INRA/Université de Bourgogne, Pôle Interaction Plantes Microorganismes, ERL6300 CNRS, Dijon, France.
FEBS Lett. 2012 Sep 21;586(19):3293-8. doi: 10.1016/j.febslet.2012.06.053. Epub 2012 Jul 13.
Endocytosis has been recently implicated in the signaling network associated with the recognition of microbes by plants. In a previous study, we showed that the elicitor cryptogein was able to induce clathrin-mediated endocytosis (CME) in tobacco suspension cells. Herein, we investigate further the induced CME by means of a GFP-tagged clathrin light chain and a CME inhibitor, the hub domain of clathrin heavy chain. Hub constitutive expression does affect neither cell growth nor constitutive endocytosis but abolishes cryptogein-induced CME. Such an inhibition has no impact on early events in the cryptogein signaling pathway but reduces the expression of defense-associated genes.
内吞作用最近被牵涉到与植物识别微生物相关的信号网络中。在之前的研究中，我们表明，激发子 cryptogein 能够诱导烟草悬浮细胞中的网格蛋白介导的内吞作用（CME）。在此，我们通过 GFP 标记的网格蛋白轻链和 CME 抑制剂——网格蛋白重链的衔接域进一步研究了诱导的 CME。衔接域组成型表达既不影响细胞生长也不影响组成型内吞作用，但会使 cryptogein 诱导的 CME 失活。这种抑制作用对 cryptogein 信号通路的早期事件没有影响，但会降低防御相关基因的表达。
